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Design of a simulator for studying eye friction and lubrication
Snopek, Lukáš ; Horák, Zdeněk (referee) ; Vrbka, Martin (advisor)
This work deals with the design of a tribometer, which simulates the interaction between the human eye and the eyelid, and simultaneously measures the friction between them and the thickness of the tear film. The tribometer will be used for research and development of artificial tears based on hyaluronic acid. The aim of the work was to design experimental conditions, conceptual and design solutions, as well as to ensure the production, assembly, and validation of the device. The experimental conditions were determined based on critical research. Four different conceptual solutions were proposed, of which two were selected for further development in the design solution. Based on the engineering documentation, the production of non-standardized components and assembly were ensured. Validation experiments demonstrated that the simulator enables the measurement of friction and tear film thickness at the contact area under the desired conditions, with both conforming and non-conforming types of contact. The contribution of this work lies in the construction of a new device that allows for measuring the coefficient of friction and film thickness during conforming contact, offering new opportunities in the study of human eye biotribology.
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Expression of endogenic lectins and their glycoligands in the tear fluid, human corneal and conjunctival epithelium under physiological and disease conditions
Hrdličková, Enkela ; Filipec, Martin (advisor) ; Heissigerová, Jarmila (referee) ; Čejková, Jitka (referee)
Purpose: Lectins play an important role in many biological processes. The aim of this work was to analyse mainly the expression of endogenic lectins, such as galectins and plant lectin, e.g. Dolichos biflorus agglutinin (DBA), and their glycoligands in the tear fluid, human corneal and conjunctival epithelium in physiological and disease conditions. Further, we studied the human natural antibody against Galα1,3Gal-R, which is mainly responsible for hyperacute rejection of xenografts transplants. We tried to investigate its localization in human corneal epithelium, lacrimal gland and tears. Material and Methods: Human tissue (lacrimal gland, tear fluid, conjunctiva, cornea, epidermis, keratinocyte and cultured corneal epithelium), as well as porcine tissue (cornea, liver and epidermis) were examined. Endogenous galectins (galectins-1, -3 and -7) were detected using immunohistochemistry methods. Binding sites for galectins, as well as binding sites for plant lectin Dolichos biflorus agglutinin, were localized by lectin histochemistry. Reverse lectin histochemistry was used for the study of binding reactivity of endogenous lectins using labelled (neo)glycoligands. Employing biotinylated natural human IgG anti -galactosides, as well as anti -galactosides, we detected reactive epitopes in human...
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Expression of endogenic lectins and their glycoligands in the tear fluid, human corneal and conjunctival epithelium under physiological and disease conditions
Hrdličková, Enkela ; Filipec, Martin (advisor) ; Heissigerová, Jarmila (referee) ; Čejková, Jitka (referee)
Purpose: Lectins play an important role in many biological processes. The aim of this work was to analyse mainly the expression of endogenic lectins, such as galectins and plant lectin, e.g. Dolichos biflorus agglutinin (DBA), and their glycoligands in the tear fluid, human corneal and conjunctival epithelium in physiological and disease conditions. Further, we studied the human natural antibody against Galα1,3Gal-R, which is mainly responsible for hyperacute rejection of xenografts transplants. We tried to investigate its localization in human corneal epithelium, lacrimal gland and tears. Material and Methods: Human tissue (lacrimal gland, tear fluid, conjunctiva, cornea, epidermis, keratinocyte and cultured corneal epithelium), as well as porcine tissue (cornea, liver and epidermis) were examined. Endogenous galectins (galectins-1, -3 and -7) were detected using immunohistochemistry methods. Binding sites for galectins, as well as binding sites for plant lectin Dolichos biflorus agglutinin, were localized by lectin histochemistry. Reverse lectin histochemistry was used for the study of binding reactivity of endogenous lectins using labelled (neo)glycoligands. Employing biotinylated natural human IgG anti -galactosides, as well as anti -galactosides, we detected reactive epitopes in human...
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